ACE-031

 This product, ACE-031 (also known as RAP-031), is a recombinant fusion protein research tool composed of the extracellular domain of the human ActRIIB receptor fused to an IgG1 Fc fragment. This compound was originally developed by Acceleron Pharma and entered Phase II clinical trials for Duchenne Muscular Dystrophy, but its clinical development has been discontinued, and it has not received marketing approval as a drug or therapy in any country. The product sold on this page is strictly and singularly defined as a “molecular probe for fundamental research on the Transforming Growth Factor-beta (TGF-β) superfamily signaling pathways”. It is not sold as a pharmaceutical, clinical research material, body enhancement agent, sports supplement, or veterinary product. Any form of human use, clinical application, veterinary use, or operation related to non-scientific purposes is strictly prohibited.

1. Product Overview
This product, ACE-031, is a recombinant fusion protein for fundamental scientific research. It is prepared as a specific molecular tool for studying TGF-β superfamily signal transduction, particularly the myostatin/GDF-8 pathway. It is produced via a mammalian cell expression system, purified, and supplied as a lyophilized powder or solution. It is specifically designed for high-level in vitro fundamental research in the fields of molecular biology, cellular signal transduction, and structural biology.

2. Key Features

• Defined Research Tool Identity: Provides a literature-based description of the protein structure (ActRIIB-Fc) and states its restriction to fundamental research purposes only.

• High Purity: Verified by SDS-PAGE and SEC-HPLC analysis, with a purity of ≥ 90% (specific to batch COA).

• Basic Activity Verification: Its in vitro binding activity to specific ligands (e.g., GDF-8) can be verified by methods such as SPR or ELISA; this activity solely demonstrates its function as a research tool.

• Storage Conditions: Lyophilized powder is recommended for storage at -20°C or below in a dry state; solution forms must be stored strictly according to COA instructions.

3. Primary Research Applications (Examples – Must be highly fundamental and mechanistic)

• Fundamental Receptor-Ligand Interaction Studies: On in vitro platforms such as Surface Plasmon Resonance or Bio-Layer Interferometry, serves as an immobilized ligand or analyte to study the binding kinetics, affinity, and specificity with members of the TGF-β superfamily (e.g., GDF-8, GDF-11).

• Cellular Signaling Pathway Inhibition Mechanism Studies: In reporter gene cell lines overexpressing related pathways, serves as a tool protein to quantitatively study the efficacy and mechanism by which it blocks ligand-induced downstream signaling (e.g., Smad phosphorylation).

• Protein Structure and Function Research: Serves as a standard protein for studying the structural properties, stability of Fc-fusion proteins, or their interaction with Protein A/G.

• Methodology Development Control: In developing assays to detect inhibitors of TGF-β superfamily signaling pathways, serves as a positive control with known activity.

4. Usage and Storage

• Strictly follow the reconstitution, dilution, and storage guidelines provided in the accompanying Certificate of Analysis.

• Avoid repeated freeze-thaw cycles. Use aseptic technique and wear appropriate personal protective equipment when handling recombinant proteins.

• Highest-Level Warning: Operators must be fully aware of this product’s special background as a discontinued clinical research compound and commit to using it solely for compliant in vitro fundamental research.

5. Disclaimer
This product is sold solely as a scientific probe for exploring fundamental biological questions. The definition “research tool” is intended to effect a complete legal and ethical separation from all clinical, enhancement, and agricultural applications. The seller does not possess the production or distribution qualifications for pharmaceuticals, biological products, or veterinary drugs. The purchaser must be a formal academic research institution, university, or non-clinical R&D department of a biotech company and must provide institutional proof at the time of purchase. The purchaser must provide a written commitment that the purchased product will be used absolutely and exclusively for legal, compliant in vitro fundamental research, and will not, under any circumstances, attempt to use it in any living being (human or animal), any clinical or preclinical therapeutic experiment, any form of physical performance enhancement, or any agriculture/livestock-related research. The seller expressly disclaims and fully excludes any and all legal liability, health risks, ethical disputes, and unlimited liability arising from the purchaser’s violation of this agreement and any misuse or abuse. This description is solely an explanation of the nature of this molecule as a research tool and does not constitute any promise of efficacy, safety, or application.